Sigma-Aldrich Cpgenome 5-Mc And 5-Hmc Dna Standard Set

CpGenome 5-mC and 5-hmC DNA Standard Set contains three linear, double-stranded DNA (897 bp) with the same sequence, but each contains 100 % 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines. This set can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications.

Materials Provided:

Three vials, each containing 2 µg (40 µL) of CpGenome Cytosine DNA Standard, CpGenome 5-mC DNA Standard, or CpGenome 5-hmC DNA Standard, at a concentration of 50 ng/μL.

Validation:

The three Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µL (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.

CpGenome is a trademark of Serologicals Corporation.

Liquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0

Recommended Storage: Stable for 6 months at -20°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.