Sigma-Aldrich Yumm 1.9 Mouse Melanoma Cell Line

Immunocompetent genetically-engineered mouse models harboring discrete genetic drivers of melanoma are essential for studying potential immunotherapies but are limited by the need to maintain colonies of multiple genotypes necessary to generate mouse models with appropriate genetic backgrounds.The YUMM (Yale University Mouse Melanoma) cell lines were developed as models that recapitulate genetic drivers found in a significant proportion of human melanomas.SourceYUMM1.9 mouse melanoma cells were derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing. References:1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.

The YUMM1.9 cell line is driven by Braf activation, Pten inactivation, and Cdkn2a inactivation. The cell line is positive for the common melanoma marker, MelanA. The cells are syngeneic with C57BL/6J mice and are very tumorigenic in vivo.

​The YUMM cell models recapitulate genetic drivers found in a significant proportion of human melanomas. YUMM1.9 cells are driven by Braf activation, Pten inactivation, and Cdkn2a inactivation. The cell line is positive for the common melanoma marker, MelanA. The cells are syngeneic with C57BL/6J mice and are very tumorigenic in vivo.

YUMM1.9 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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