Sigma-Aldrich Isgec Pss1 Hu Salivary Gland Cell Line

Primary Sjögren′s syndrome (PSS), a chronic autoimmune disorder, causes a variety of problems in patients such as dry mouth, dry eyes, and other problems relating to lacrimal and salivary gland disfunction which are often known as sicca symptoms. The prevalence of PSS is hard to understand due to continual changes in disease criteria. It is still considered a highly relevant disease to understand and is considered the second most common autoimmune disease. PSS typically affects females in their 40s or 50s. Criteria for classification of PSS are based on a sum of factors such as unstimulated whole saliva flow rate and the presence of anti-Ro/SSA antibodies. Neurological conditions are also common features of PSS although their manifestations vary. Although PSS is commonly associated with its sicca related symptoms, some patients do not present sicca symptoms and may never develop them in their disease progression and manifestation.The potential therapies for salivary gland disfunction remain rather limited. This is likely due to the lack of cell models that appropriately represent the related diseases such as PSS and sicca.1 Researchers often rely on cancer cell lines or short-term cultures of primary salivary gland epithelial cells (SGECs). The iSGEC (immortalized Salivary Gland Epithelial Cell) group of cell lines appear to be the first reliable immortalized human primary SGEC lines derived from multiple female patient′s labial salivary gland biopsies. These cell lines were developed to mimic conditions of salivary gland disfunction. The pSS1 cell line was developed from a salivary gland biopsy of a patient who was affected by Primary-Sjögren′s Syndrome (PSS). The cell lines were characterized for pro-acinar and epithelial cell markers and are expected to secrete α-amylase upon β-adrenergic stimulation. The iSGEC group of cell lines can also be grown as a 3D culture.Source GMO; Derived from the salivary gland biopsy of a patient with Primary-Sjögren′s Syndrome (PSS). Cells were later immortalized with SV40 Large-T antigen.References1. Noll BD, Grdzelishvili A, Brennan MT, Mougeot FB, Mougeot J-LC. 2020. Immortalization of Salivary Gland Epithelial Cells of Xerostomic Patients: Establishment and Characterization of Novel Cell Lines. Journal of Clinical Medicine. 9(12):3820. 2. Neumann M, Quintero J, Shih T, Capitle EM. 2021 Jan 29. Not all Sicca is Sjögren′s and not all Sjögren′s is Sicca. Cureus.

The iSGEC-pSS1 human salivary gland epithelial Sjogren′s Syndrome cell line was developed from a salivary gland biopsy of a patient who was affected by Primary-Sjögren′s Syndrome (PSS).

iSGEC-pSS1 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.

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