Sigma-Aldrich Anti-Gtf2B Clone 1J4 Zoomab Rb Mono

Transcription initiation factor IIB (UniProt: Q00403; also known as EC:2.3.1.48, General transcription factor TFIIB, S300-II) is encoded by the GTF2B (also known as TF2B, TFIIB) gene (Gene ID: 2959) in human. Transcription initiation factor IIB (GTF2B) is a critical component of the transcription machinery, playing a pivotal role in the formation of the pre-initiation complex (PIC) required for the transcription of genes by RNA polymerase II. GTF2B interacts with the TATA-binding protein (TBP) and RNA polymerase II, facilitating the recruitment and stabilization of the transcriptional machinery at the promoter region. This protein is involved in recognizing the BRE (TFIIB recognition element) sequence, thereby influencing the selection of transcription start sites. GTF2B is ubiquitously expressed across various tissues, reflecting its essential role in general transcriptional processes. GTF2B is localized in the nucleus. Dysregulation of GTF2B has been implicated in various diseases, including cancer, where alterations in transcriptional control can lead to aberrant gene expression profiles. Recent studies have explored the potential of targeting GTF2B in therapeutic interventions, particularly in tumors with specific transcriptional dependencies. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ha, I., et al., (1991), Cell, 66(3), 551-561; Deng, W., et al., (2018), Nature Communications, 9(1), 543; Zhang, Y., et al., (2021), Cancer Research, 81(23), 5902-5914).

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal half of human GTF2B.

Quality Control Testing

Evaluated by Western Blotting in nuclear extract from HEK-293 cells.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected GTF2B in nuclear extract from HEK-293 cells.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound with GTF2B peptide with at least one million-fold (1,000,000X) higher affinity than with non-specific control peptide.

Immunocytochemistry Analysis: A 1:1,000 Dilution from a representative lot detected GTF2B in HEK-293 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GTF2B in human small intestine tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1J4 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects GTF2B. It targets an epitope within 24 amino acids from the N-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.