Sigma-Aldrich Anti-Merlin Clone 1H15-L1 Zoomab Rb

Merlin (UniProt: P35240; also known as Moesin-ezrin-radixin-like protein, Neurofibromin-2, Schwannomerlin, Schwannomin) is encoded by the NF2 (also known as SCH) gene (Gene ID: 4771) in human. Originally identified as a tumor suppressor gene in the familial Neurofibromatosis type 2 (NF2) cancer patients, NF2 encodes the FERM (Ezrin, Radixin, Moesin) domain-containing merlin that plays a negative regulating role against cell growth and proliferation by functioning as an upstream activator of the Hippo pathway. Merlin directly activates Mst1/2 and recruits Lats1/2 to the plasma membrane for phosphorylation by Mst1/2. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Subcellularly, Merlin localizes to the plasma membrane, cytoplasm, and nucleus, where it can interact with components of the Hippo signaling pathway, thereby regulating cell growth and tissue homeostasis. Two predominant isoforms (1 & 3) and a number of minor isoforms are produced by alternatively spliced transcripts. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Dysregulation of Merlin′s function can also lead to oncogenic processes, particularly in meningiomas, where loss of Merlin expression results in the activation of Yes-associated protein (YAP), a key regulator of cell proliferation and survival. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Goda, N., et al. (2024). Development. 151(14):dev202639; Tanahashi, K., et al. (2015). J Neuropath. Exp Neurol. 74(7):704-9).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal half of human Merlin.

Quality Control Testing

Evaluated by Western Blotting in PC-3 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Merlin in PC-3 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Merlin in lysate from mouse brain tissue and rat brain tissue and, a 1:10,000 dilution from a representative lot detected Merlin in lysate from SH-SY5Y cells and human brain tissue.

Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated Merlin in PC-3 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound Merlin peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1H15-L1 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects Merlin. It targets an epitope within 25 amino acids from the C-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.