Sigma-Aldrich Anti-Cx3Cr1 Clone 1N5 Zoomab Rb Mono

CX3C chemokine receptor 1 (UniProt: P49238; also known as CX3C chemokine receptor 1, C-X3-C CKR-1, Beta chemokine receptor-like 1, CMK-BRL-1, Fractalkine receptor, G-protein coupled receptor 13, V28) is encoded by the CX3CR1 (also known as CMKBRL1, GPR13) gene (Gene ID: ) in human. CX3CR1, or C-X3-C motif chemokine receptor 1, is a G protein-coupled receptor that primarily binds to the chemokine fractalkine (CX3CL1). This receptor is predominantly expressed on various immune cells, including natural killer cells, monocytes, and subsets of T cells, playing crucial roles in mediating cell adhesion and migration through its interaction with fractalkine. CX3CR1 is characterized by seven transmembrane domains typical of chemokine receptors. CX3CR1 is localized to the plasma membrane, where it orchestrates immune surveillance and inflammatory responses by recruiting leukocytes to sites of tissue injury or infection. The expression of CX3CR1 is notably high in the brain, particularly in microglia, implicating its involvement in neuroinflammatory processes. Dysregulation of CX3CR1 signaling has been linked to various pathological conditions, including atherosclerosis, where it contributes to monocyte recruitment to atherosclerotic plaques, and neurodegenerative diseases like Alzheimer′s, where it influences microglial activation and neuroinflammation. Recent studies have also highlighted its potential as a therapeutic target in cancer, given its role in tumor cell migration and immune cell infiltration. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lee, M., et al., (2018), Immune Netw, 18(1), e5; von Vietinghoff, S., et al., (2021), Cell Tissue Res, 384(2), 401-410; Kim, K.W., et al., (2011), Blood, 118(11), 2883-2894).

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal extracellular domain of human CX3CR1.

Quality Control Testing

Evaluated by Western Blotting in LNCaP cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CX3CR1 in LNCaP cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CX3CR1 in lysates from Raji membrane, Jurkat membrane, human brain cortex tissue, and THP-1 cells.

Immunocytochemistry Analysis (ICC): A 1:100 dilution from a representative lot detected CX3CR1 in unpermeabilized RPMI 8226 cells.

Affinity Binding Assay: A representative lot of this antibody bound with CX3CR1 peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1N5 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects CX3CR1. It targets an epitope within 17 amino acids from the N-terminal, extracellular domain.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.