Sigma-Aldrich Anti-Pck2/Pepck Clone 2I12 Zoomab Rb

Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (UniProt: Q16822; also known as EC:4.1.1.32, PEPCK-M, Phosphoenolpyruvate carboxykinase 2, mitochondrial, mtPCK2) is encoded by the PCK2 (also known as PEPCK2) gene (Gene ID: 5106) in human. Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PCK2) is a crucial enzyme in gluconeogenesis, catalyzing the conversion of oxaloacetate to phosphoenolpyruvate, a key step in glucose production from non-carbohydrate precursors. PCK2 is a homodimeric protein characterized by a unique GTP-binding domain that distinguishes it from its cytosolic counterpart, PCK1. This protein is predominantly localized in the mitochondria, facilitating its role in integrating metabolic pathways between the mitochondria and cytosol. PCK2 is expressed in various tissues, with particularly high levels in the liver and kidney, reflecting its importance in metabolic regulation. It interacts with several proteins, including components of the mitochondrial transport machinery and other metabolic enzymes, enhancing its regulatory capacity in gluconeogenesis and the tricarboxylic acid (TCA) cycle. Dysregulation of PCK2 has been implicated in metabolic disorders, such as diabetes and cancer, where altered gluconeogenic flux contributes to disease progression. Recent studies have shown that PCK2 may support cancer cell survival under glucose deprivation. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Brearley, M.C., et al., (2020), Sci Rep 10, 22177; Ye, Q., et al., (2023) Nat Commun 14, 1402).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal of human PCK2/PEPCK.

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PCK2/PEPCK in HepG2 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PCK2/PEPCK in LNCaP cell lysate and a 1:10,000 dilution in mouse liver and rat liver tissue lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PCK2/PEPCK in HepG2 cells.

Affinity Binding Assay: A representative lot of this antibody bound with PCK2/PEPCK peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 2I12 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects PCK2/PEPCK. It targets an epitope within 25 amino acids from the C-terminal.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.