Sigma-Aldrich RPMI-1640 Medium. 10 L

Name: Sigma-Aldrich RPMI-1640 Medium. 10 L
SKU: SIAL-R4130-10L
Price: 145.20 USD

List Price $145.20 Your Price $145.20

Sigma-Aldrich RPMI-1640 Medium. HEPES Modification, with L-glutamine and 25mM HEPES, without sodium bicarbonate, powder, suitable for cell culture. 10 L - SIAL (Additional S&H Or Hazmat Fees May Apply)

NETA PART: SIAL-R4130-10L

MFG.PART: R4130-10L

UNSPSC: 41122100

Manufacturer: Sigma-Aldrich

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Description

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
NaHCO₃: no
phenol red: yes
HEPES: 25 mm
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

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Related Categories

Cell Culture & Analysis

Cell Culture Media & Buffers

Classical Media & Buffers

Molecular Biology & Functional Genomics

RPMI 1640 Media

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Preparation Note

Formulated to contain 16.4 grams of powder per liter of medium.

Supplement with 2.0 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c