Sigma-Aldrich Trypsin inhibitor. powder. 250 mg

Name: Sigma-Aldrich Trypsin inhibitor. powder. 250 mg
SKU: SIAL-T9253-250MG
Price: 85.50 USD

List Price $85.50 Your Price $85.50

Sigma-Aldrich Trypsin inhibitor. powder. 250 mg - SIAL (Additional S&H Or Hazmat Fees May Apply)

NETA PART: SIAL-T9253-250MG

MFG.PART: T9253-250MG

UNSPSC: 12352200

Manufacturer: Sigma-Aldrich

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Description

Product Name

Trypsin inhibitor from chicken egg white, Type II-O, Partially purified ovomucoid, containing ovoinhibitor

biological source

chicken egg white

type

Type II-O

form

powder

mol wt

28 kDa

solubility

water: soluble 10 g/L

storage temp.

2-8°C

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General description

25% of ovomucoid comprises carbohydrate.[1]

Ovomucoid or trypsin inhibitor is an abundant protein in most avian egg whites. The hen′s egg protein is composed of about 186 amino acid residues, and is highly glycosylated. Three tandem domains are each homologous with pancreatic trypsin inhibitor. It is highly immunogenic, and probably accounts for most cases of egg allergy.

Application

Trypsin inhibitor from chicken egg white has been used:

to produce denatured, oxidized and deglycosylated ovomucoid and used to stimulate patient derived cell cultures for mapping T cell epitopes[1]
to treat nuclei for flow cytometric analysis[2]
in Holtfreter′s solution, used for the dissociation of planarians into cells[3]

Analysis Note

One mg will inhibit 0.8-1.6 mg of tryspin with activity of approx. 10,000 BAEE units per mg protein. May inhibit ≤0.3 mg of chymotrypsin with activity of approx. 40 BTEE units per mg protein.

Other Notes

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Ovomucoid, from chicken egg white, does not itself inhibit chymotrypsin. Chymotrypsin inhibition is a measure of ovoinhibitor contamination by method of Feeney, R. E., et Al., J. Biol. Chem., 238, 1415 (1963).

View more information on Trypsin Inhibitor.

pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c