Sigma-Aldrich Cytochrome c from bovine heart. ≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture. 50 mg

Name: Sigma-Aldrich Cytochrome c from bovine heart. ≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture. 50 mg
SKU: SIAL-C3131-50MG
Price: 180.96 USD

List Price $180.96 Your Price $180.96

Sigma-Aldrich Cytochrome c from bovine heart. ≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture. 50 mg - SIAL (Additional S&H Or Hazmat Fees May Apply)

NETA PART: SIAL-C3131-50MG

MFG.PART: C3131-50MG

UNSPSC: 41122100

Manufacturer: Sigma-Aldrich

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Description

biological source

bovine heart

assay

≥95% based on Mol. Wt. 12,327 basis

form

powder

mol wt

12327 Da

storage condition

(Tightly closed Dry)

technique(s)

cell culture | mammalian: suitable

impurities

0.40-0.50% Iron (anhydrous)

solubility

water: 10 mg/mL, dark red-brown

UniProt accession no.

P62894

storage temp.

−20°C

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General description

Research area: Apoptosis

Cytochrome c (Cyt C) is an essential mitochondrial protein located in the inner membrane of the mitochondria.[1] It is encoded in the nucleus as apo-cytochrome C.[2] Cyt C occurs as membrane-bound or as soluble metalloproteins in energy-transducing membranes and is found in bacteria, archaea, and plastids.[1] It belongs to the group of class IIa cytochromes and contains pentacoordinate heme centres.[3]

Application

Cytochrome C from bovine heart has been used:

as a component of the assay solution for testing the activity of respiratory chain complexes III and IV in the mitochondria of human osteosarcoma and human hepatocarcinoma cell lines[4]
to determine the cytochrome c oxidase activity in mitochondria of human aortic endothelial cells[5]
as a component of the cyt C oxidase reaction solution during in situ enzyme staining of cyt C oxidase in mice kidney tissues[6]

Biochem/physiol Actions

Cytochrome C (Cyt C) is involved in the activation of caspase during the caspase-dependent apoptosis intrinsic pathway that triggers programmed cell death through apoptosis.[7] It shows lipid-binding activity through its interaction with cardiolipin that accounts for the peroxidase activity of Cyt C.[1] Cyt C binds to heme via the help of cytochrome c heme lyase that enables its release into the mitochondrial intermembrane space.[8]

The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen.

The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen.

Preparation Note

Prepared with acetic acid without using TCA.

Other Notes

View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c