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Sigma-Aldrich Prolidase from porcine kidney. lyophilized powder, ≥100 units/mg protein. 250 units
List Price $455.88 Your Price $455.88
Sigma-Aldrich Prolidase from porcine kidney. lyophilized powder, ≥100 units/mg protein. 250 units - SIAL (Additional S&H Or Hazmat Fees May Apply)
NETA PART: SIAL-P6675-250UN
MFG.PART: P6675-250UN
UNSPSC: 12352204
Manufacturer: Sigma-Aldrich
form
lyophilized powder
specific activity
≥100 units/mg protein
composition
Protein, 20-74% Lowry
storage temp.
−20°C
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Related Categories
Proteases
Proteins & Enzymes
General description
Prolidase is a cytosolic exopeptidase.[1] It is a homodimeric enzyme which requires divalent cations like manganese as a cofactor in its active site for its function.[2]
Application
Prolidase from porcine kidney has been used:
- in the enzymatic hydrolysis of porcine milk for the recovery of L-glutamine from proteins and peptides[3]
- in the proteolysis of skim milk for the determination of ε-(γ-glutamyl)lysine and free aminoacids[4]
- to determine its effect on the activity of enterococcin A 2000[5]
Prolidase has an important role in recycling of proline and collagen production. It is used to study mutations in the PEPD gene that cause prolidase deficiency. It is used to hydrolyze proteins with C-terminal proline or hydroxyproline residues. Prolidase, product P6675 from porcine kidney, has been used to hydrolyze peptide bonds from the amino terminus when studying enzymatic methylation of membrane proteins[6].
Biochem/physiol Actions
Prolidase is an enzyme that catalyzes the hydrolysis of the imide bond between an α-carboxyl group and proline or hydroxyproline. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues.
Rare mutation in prolidase gene causes deficiency leading to massive imidodipeptiduria, elevated proline-containing dipeptides in plasma, recurrent infections, mental retardation and skin lesions.[1][2]
Physical form
Supplied as a lyophilized powder containing Tris buffer salt and MnCl2.
Other Notes
One unit will hydrolyze 1.0 μmole of Gly-Pro per min at pH 8.0 at 40 °C.
Pictograms
GHS08,GHS07
signalword
Danger
hcodes
H315,H319,H334,H335
pcodes
P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
| SKU | SIAL-P6675-250UN |
|---|---|
| Supplier Part Number | P6675-250UN |
| UM | EA |
| UNSPSC | 12352204 |
| Manufacturer | Sigma-Aldrich |
| Temperature | -20C |
| Refrigerated-Frozen | Y |
| ProductLine | SIAL |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 10.000000 |
| Lead Time | 9 |
| CAS Number | 9025-32-5 |
| Energy Star | No |
| Green | No |
| Controlled | N |