Sigma-Aldrich Glutaminase from Escherichia coli. Grade V, lyophilized powder, ≥50 units/mg protein. 10 units

Name: Sigma-Aldrich Glutaminase from Escherichia coli. Grade V, lyophilized powder, ≥50 units/mg protein. 10 units
SKU: SIAL-G8880-10UN
Price: 214.60 USD

List Price $214.60 Your Price $214.60

Sigma-Aldrich Glutaminase from Escherichia coli. Grade V, lyophilized powder, ≥50 units/mg protein. 10 units - SIAL (Additional S&H Or Hazmat Fees May Apply)

NETA PART: SIAL-G8880-10UN

MFG.PART: G8880-10UN

UNSPSC: 12352204

Manufacturer: Sigma-Aldrich

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Description

biological source

Escherichia coli

Quality Level

200

type

Grade V

form

lyophilized powder

specific activity

≥50 units/mg protein

mol wt

110 kDa

composition

Protein, ~30% biuret

solubility

water: 1 mg/mL (Clear W/Particle to Sl. Hazy W/Particles)

cation traces

NH₄⁺: <0.1 μg/unit

foreign activity

NADH Oxidase ≤0.01%

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General description

Glutaminase is an amidohydrolase.[1] It has the catalytic domain in the N-terminal and the C-terminal extension possesses STAS domain.[2]

Application

Glutaminase from E.coli has been used:

for the determination of glutamine from the cerebral cortex tissue extract[3]
for immobilization on aminopropylsilylated controlled pore glass (CPG) for determination of glutamine[1]
in combination with acivicin in in vitro melanoma cells[4] and MCF-7 and OAW-42 cells[5] for cell proliferation and invasiveness assay.

Biochem/physiol Actions

Glutaminase catalyzes the conversion of glutamine to glutamate.

Glutaminase from Escherichia coli along with acivicin, a glutamine analog regulates proliferation and invasiveness of cancer cells.[4][5] Bacterial glutaminase is implicated in increasing the life span of cancer patients by lowering the tumor burden.[5]

Physical form

Lyophilized powder containing potassium succinate and EDTA

Other Notes

One unit will deaminate 1.0 μmole of L-glutamine per min at pH 4.9 at 37 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c