Sigma-Aldrich 3-Methyladenine. ≥99% (HPLC), synthetic (organic), powder. 100 mg

Name: Sigma-Aldrich 3-Methyladenine. ≥99% (HPLC), synthetic (organic), powder. 100 mg
SKU: SIAL-M9281-100MG
Price: 310.88 USD

List Price $310.88 Your Price $310.88

Sigma-Aldrich 3-Methyladenine. ≥99% (HPLC), synthetic (organic), powder. 100 mg - SIAL (Additional S&H Or Hazmat Fees May Apply)

NETA PART: SIAL-M9281-100MG

MFG.PART: M9281-100MG

UNSPSC: 12352200

Manufacturer: Sigma-Aldrich

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Description

Product Name

3-Methyladenine, autophagy inhibitor

biological source

synthetic (organic)

Quality Level

300

assay

≥99% (HPLC)

form

powder

mp

~300 °C (dec.) (lit.)

solubility

DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow

storage temp.

room temp

SMILES string

Cn1cnc(N)c2ncnc12

InChI

1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3

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Related Categories

Biochemicals

Chemistry & Biochemicals

Nucleosides and Nucleotides

Application

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mm.

3-Methyladenine has been used as an autophagy inhibitor:

to determine the expressions of autophagy-related genes (Beclin 1, light chain 3 (LC3) and p62) using the transfected human bone marrow mesenchymal stem cells (BM-MSCs)[1]
with cisplatin to study the inhibition of autophagy influenced cisplatin-induced apoptosis in A2780cp cells[2]
to study the interplay between colistin-induced autophagy and apoptosis[3]

Standard for detection of nucleic acid methylation during carcinogenesis.

Biochem/physiol Actions

Preparation Note

This product is soluble in DMF (10 mg/mL; may require gentle heating). It is also soluble in water, 95% ethanol, or 1 N NaOH at 30 mg/mL with heating, but upon cooling, the product precipitates from solution. Solutions of 3-MA may be prepared in DMSO at 100 mm.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c