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Sigma-Aldrich Monoclonal Anti-polyHistidine antibody produced in mouse. clone HIS-1, ascites fluid. 100 μL
List Price $618.27 Your Price $618.27
Sigma-Aldrich Monoclonal Anti-polyHistidine antibody produced in mouse. clone HIS-1, ascites fluid. 100 μL - SIAL (Additional S&H Or Hazmat Fees May Apply)
NETA PART: SIAL-H1029-100UL
MFG.PART: H1029-100UL
UNSPSC: 12352203
Manufacturer: Sigma-Aldrich
biological source
mouse
Quality Level
200
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
HIS-1, monoclonal
form
liquid
technique(s)
DOT blot: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotype
IgG2a
shipped in
dry ice
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General description
The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors PET, pRSET, and pTrc.
Immunogen
recombinant polyHistidine tagged fusion protein.
Application
Antibody suitable for immunoblotting, DOT blot assays, ELISA and immunocytochemistry. Monoclonal Anti-polyHistidine antibody produced in mouse has been used in Western blotting.
Biochem/physiol Actions
The IgG2a (Immunoglobulin g 2a) gene actively takes part in stimulating autoimmunity in mice. The gene is found to be highly expressed in virus infection.
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
Physical form
Suppled as ascites fluid with 15mM sodium azide.
Other Notes
Recommended dilution for immunoblotting is 1:3000
Legal Information
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
| SKU | SIAL-H1029-100UL |
|---|---|
| Supplier Part Number | H1029-100UL |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIAL |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Energy Star | No |
| Green | No |
| Controlled | N |