Sigma-Aldrich Collagenase from Clostridium histolyticum. 100 mg

Name: Sigma-Aldrich Collagenase from Clostridium histolyticum. 100 mg
SKU: SIAL-C2139-100MG
Price: 158.92 USD

List Price $158.92 Your Price $158.92

Sigma-Aldrich Collagenase from Clostridium histolyticum. 100 mg - SIAL (Additional S&H Or Hazmat Fees May Apply)

NETA PART: SIAL-C2139-100MG

MFG.PART: C2139-100MG

UNSPSC: 41116113

Manufacturer: Sigma-Aldrich

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Description

biological source

Clostridium histolyticum

Quality Level

200

type

Type VIII

form

powder

specific activity

≥125 CDU/mg solid
0.5-5.0 FALGPA units/mg solid

mol wt

68-125 kDa

suitability

suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV)

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

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Related Categories

Collagenase

Protein Biology

Proteins & Enzymes

Tissue Dissociation

General description

Clostridium histolyticum is a pathogenic clostridium that produces crude collagenases. This is a mixture of enzymes containing collagenase, non-specific proteases and clostripain.[1]

Application

Collagenase may be used:[2][1][3][4][5][6]

for the preparation of arterial tissue for the study of advanced glycosylation end products (AGE)
for use along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues.
in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes.
for the preparation of viable hepatocytes from rat liver and for the isolation of fat cells from rat adipose tissue

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(β-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, β-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline. Collagenase enzymes and neutral protease plays an important role in the effective release of cells from tissue.[7] Collagenase recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X represents a neutral amino acid. [8]

Analysis Note

Also contains clostripain, nonspecific neutral protease and tryptic activities.

Other Notes

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c