Sigma-Aldrich Tris-EDTA buffer solution. BioUltra, Molecular Biology, pH 8.0. 500 mL

Name: Sigma-Aldrich Tris-EDTA buffer solution. BioUltra, Molecular Biology, pH 8.0. 500 mL
SKU: SIAL-93283-500ML
Price: 133.40 USD

List Price $133.40 Your Price $133.40

Sigma-Aldrich Tris-EDTA buffer solution. BioUltra, Molecular Biology, pH 8.0. 500 mL - SIAL (Additional S&H Or Hazmat Fees May Apply)

NETA PART: SIAL-93283-500ML

MFG.PART: 93283-500ML

UNSPSC: 12161700

Manufacturer: Sigma-Aldrich

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Description

grade

Molecular Biology

Quality Level

200

product line

BioUltra

form

liquid

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

8.0±0.2 (25 °C)

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
CR: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm A_max: 0.04
λ: 280 nm A_max: 0.01

application(s)

sample preparation

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General description

Tris EDTA is a biological buffer in research. It is a combination of Tris base and EDTA, a metal chelator. Tris provides buffering capacity, maintaining a stable pH in biological samples, while EDTA helps prevent metal ion interference by chelating divalent metal ions. Tris EDTA is often used in molecular biology techniques, such as DNA and RNA sample preparation, electrophoresis, and enzymatic reactions. It helps maintain the stability and integrity of nucleic acids by inhibiting the activity of nucleases and metal-dependent enzymes.

Application

TE buffer is useful for general extraction, suspension, washing, and storage buffer and in the preparation of DNA or RNA. Commonly used for DNA sequencing, restriction enzyme digestion; ligation and polymerase chain reaction (PCR) applications.

Features and Benefits

BioUltra grade - suitable for many molecular biology applications
DNase, RNase, phosphatase, and protease-free
High purity and free of trace metals

Other Notes

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c