Sigma-Aldrich α-Mannosidase from Canavalia ensiformis (Jack bean). ammonium sulfate suspension, ≥15 units/mg protein (biuret). 1 mg

Name: Sigma-Aldrich α-Mannosidase from Canavalia ensiformis (Jack bean). ammonium sulfate suspension, ≥15 units/mg protein (biuret). 1 mg
SKU: SIAL-M7257-1MG
Price: 205.32 USD

List Price $205.32 Your Price $205.32

Sigma-Aldrich α-Mannosidase from Canavalia ensiformis (Jack bean). ammonium sulfate suspension, ≥15 units/mg protein (biuret). 1 mg - SIAL (Additional S&H Or Hazmat Fees May Apply)

NETA PART: SIAL-M7257-1MG

MFG.PART: M7257-1MG

UNSPSC: 12352204

Manufacturer: Sigma-Aldrich

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Description

biological source

Canavalia ensiformis

form

ammonium sulfate suspension

specific activity

≥15 units/mg protein (biuret)

foreign activity

β-galactosidase, β-N-acetylglucosaminidase, α-galactosidase and α-L-fucosidase <0.05%

storage temp.

2-8°C

SMILES string

[Zn+2]

InChI

1S/Zn/q+2

InChI key

PTFCDOFLOPIGGS-UHFFFAOYSA-N

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General description

α−Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa. [1]

α-Mannosidase is an exo-glycosidase and an inc metalloenzyme.[2]

Lysosomal α-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins. [3]

Application

α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice. [4]

α-Mannosidase from Canavalia ensiformis (Jack bean) has been used:

for the digestion of neutral glycans.[5]
to remove O-glycans from recombinant β-glucosidase[6]
for the inhibition of renal fibroblasts proliferation[7]

Liberates mannose from a variety of synthetic and natural α-mannosides.

Biochem/physiol Actions

α−Mannosidase has been shown to inhibit the proliferation of B-lymphocytes. [1]

α-Mannosidase cleaves near α1−2-, α1−3- and α1−6-linked mannose residues.[2]

α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins.

Physical form

Suspension in 3.0 M (NH₄)₂SO₄ and 0.1 mm zinc acetate, pH 7.5

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH 4.5 at 25°C.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c