Sigma-Aldrich Neocarzinostatin. from Streptomyces carzinostaticus, ≥90% (SDS-PAGE), solution. 100 μg

Name: Sigma-Aldrich Neocarzinostatin. from Streptomyces carzinostaticus, ≥90% (SDS-PAGE), solution. 100 μg
SKU: SIAL-N9162-100UG
Price: 661.20 USD

List Price $661.20 Your Price $661.20

Sigma-Aldrich Neocarzinostatin. from Streptomyces carzinostaticus, ≥90% (SDS-PAGE), solution. 100 μg - SIAL (Additional S&H Or Hazmat Fees May Apply)

NETA PART: SIAL-N9162-100UG

MFG.PART: N9162-100UG

UNSPSC: 12352200

Manufacturer: Sigma-Aldrich

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Description

Product Name

Neocarzinostatin from Streptomyces carzinostaticus, ≥90% (SDS-PAGE), ~0.5 mg/mL

Quality Level

200

assay

≥90% (SDS-PAGE)

form

solution

concentration

~0.5 mg/mL

antibiotic activity spectrum

neoplastics

mode of action

DNA synthesis | interferes

shipped in

wet ice

storage temp.

2-8°C

SMILES string

N([C@H]1[C@H](O[C@@H]([C@@H]([C@@H]1O)O)C)O[C@H]2[C@@H](C=C5C2CC#C[C@H]6O[C@]6(C#C5)[C@@H]7OC(=O)OC7)OC(=O)c3c4c(c(cc(c4)OC)C)ccc3O)C

Application

Neocarzinostatin from Streptomyces carzinostaticus has been used:

as a DNA damaging agent to treat human fibroblast GM0637 cells[1]
as a radiomimetic antibiotic to induce DNA damage[2]
in combination with Vγ2Vδ2 T cells to determine the cytotoxicity in ovarian cancer cells[3]

Biochem/physiol Actions

Neocarzinostatin is a protein-small molecule complex composed of an enediyne chromophore tightly bound to a 113 amino acid single chain protein. The complex possesses antiproliferative and antitumor activity. The chromophore is the active compound, which is responsible for DNA cleavage; while the apoprotein stabilizes and regulates the availability of the labile chromophore. NCS chromophore is bound non-covalently in a cleft of the binding protein andis dissociable. Upon addition of a thiol, the chromophore forms a highly reactive biradical species that can induce sequence-specific single and double strand breaks in DNA. Neocarzinostatin inhibits DNA synthesis and possesses antitumor activity in various human and animal tumors. NCS inhibits cellular proliferation by inducing g₂ cell cycle arrest and apoptosis in both human papillomavirus (HPV) positive and negative cell lines.

Neocarzinostatin is a radiomimetic antibiotic substance.[2]

Protein-small molecule complex composed of an enediyne chromophore tightly bound to a 113-amino acid single chain protein. Chemotherapeutic agent, which inhibits DNA synthesis, possesses antitumor activity, and induces apoptosis.

Physical form

Supplied as 100 μg (~9 nmoles) in a solution containing 20 mm MES buffer, pH 5.5.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c