Sigma-Aldrich Nitroreductase from Escherichia coli. ≥90% (SDS-PAGE), recombinant, expressed in E. coli. 1 mg

Name: Sigma-Aldrich Nitroreductase from Escherichia coli. ≥90% (SDS-PAGE), recombinant, expressed in E. coli. 1 mg
SKU: SIAL-N9284-1MG
Price: 502.28 USD

List Price $502.28 Your Price $502.28

Sigma-Aldrich Nitroreductase from Escherichia coli. ≥90% (SDS-PAGE), recombinant, expressed in E. coli. 1 mg - SIAL (Additional S&H Or Hazmat Fees May Apply)

NETA PART: SIAL-N9284-1MG

MFG.PART: N9284-1MG

UNSPSC: 41122409

Manufacturer: Sigma-Aldrich

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Description

recombinant

expressed in E. coli

Quality Level

200

assay

≥90% (SDS-PAGE)

form

lyophilized powder

specific activity

≥100 units/mL

mol wt

monomer 24000

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UniProt accession no.

P38489

greener alternative category

, Re-engineered

General description

Lyophilized powder containing PBS. Does not contain BSA as excipient

Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.[1]

Application

Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE).[2] It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.[3]

Biochem/physiol Actions

Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones.[1] The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.[4]

Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical. [5]

Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant. [6]

Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.

Preparation Note

Produced using animal component-free materials.

Other Notes

One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c