Sigma-Aldrich N,N,N′,N′-Tetrakis(2-pyridylmethyl)ethylenediamine. ≥98% (TLC), powder, membrane-permeable zinc chelator. 100 mg

N,N,N′,N′-Tetrakis(2-pyridylmethyl)ethylenediamine has been used:

• in the treatment of human embryonic kidney (HEK293T) cells for assessing its effect on the Jembrana disease virus (JDV) viral infectivity factor (Vif) functionality[1]
• in P19 embryonic carcinoma cells for chelation of zinc ions released during apoptosis[2]
• to evaluate its effect on lentiviral Vpx infection on human monocytic THP-1 cells[3]

N,N,N′,N′-Tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) elicits pro-apoptotic effect in human acute promyelocytic NB4 cells by inhibiting nitric oxide signalling and Zn²⁺ signaling.[4] In microglia pretreated with zinc, TPEN inhibits the interleukin 6 secretion and nitric oxide synthase (iNOS) mRNA expression.[5] It induces damage on DNA structure by activating ATM (ataxia telangiectasia mutated) and ATR (ATM-Rad3-related) signaling in human colon cancer cells.[6]

TPEN is a membrane-permeable zinc chelator; decreased the intracellular level of zinc and induced apoptosis (i.e., cell shrinkage and formation of apoptotic bodies with DNA fragmentation and formation of a typical DNA ladder pattern).

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