Sigma-Aldrich BCIP/NBT-Blue. alkaline phosphatase substrate, chromogenic, liquid. 100 mL

Name: Sigma-Aldrich BCIP/NBT-Blue. alkaline phosphatase substrate, chromogenic, liquid. 100 mL
SKU: SIAL-B3804-100ML
Price: 179.80 USD

List Price $179.80 Your Price $179.80

Sigma-Aldrich BCIP/NBT-Blue. alkaline phosphatase substrate, chromogenic, liquid. 100 mL - SIAL (Additional S&H Or Hazmat Fees May Apply)

NETA PART: SIAL-B3804-100ML

MFG.PART: B3804-100ML

UNSPSC: 41116127

Manufacturer: Sigma-Aldrich

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Description

Product Name

BCIP^®/NBT-Blue Liquid Substrate System for Membranes, alkaline phosphatase substrate

Quality Level

100

form

liquid

storage temp.

2-8°C

Related Categories

Detection Substrates & Enzymes

Protein Biology

Proteins & Enzymes

General description

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP^®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.

Application

BCIP^®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity[1][2] such as western blot analysis.[3]

Other Notes

Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Stable at least one year at 2-8 °C. This substrate is light-sensitive.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c