We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn about our cookie policy.
Sigma-Aldrich 4-Nitrophenyl phosphate di(Tris) salt. phosphatase substrate, ≥97% (enzymatic), powder. 5 g
List Price $122.96 Your Price $122.96
Sigma-Aldrich 4-Nitrophenyl phosphate di(Tris) salt. phosphatase substrate, ≥97% (enzymatic), powder. 5 g - SIAL (Additional S&H Or Hazmat Fees May Apply)
NETA PART: SIAL-N3254-5G
MFG.PART: N3254-5G
UNSPSC: 41116127
Manufacturer: Sigma-Aldrich


Product Name
4-Nitrophenyl phosphate di(Tris) salt, phosphatase substrate
assay
≥97% (enzymatic)
form
powder
solubility
water: 50 mg/mL, clear, colorless to light yellow
storage temp.
−20°C
SMILES string
NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI
1S/C6H6NO6P.2C4H11NO3/C8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
InChI key
XXAXKCWOTRABOW-UHFFFAOYSA-N
Looking for similar products? Visit Product Comparison Guide
Related Categories
Chemistry & Biochemicals
Detection Substrates & Enzymes
Phosphates
Protein Biology
Proteins & Enzymes
Application
4-Nitrophenyl phosphate di(Tris) salt has been used:
- as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates[1]
- to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)[2]
- for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)[2]
Biochem/physiol Actions
p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.[3]
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
PPE
Eyeshields, Gloves, type N95 (US)
| SKU | SIAL-N3254-5G |
|---|---|
| Supplier Part Number | N3254-5G |
| UM | EA |
| UNSPSC | 41116127 |
| Manufacturer | Sigma-Aldrich |
| Temperature | -20C |
| Refrigerated-Frozen | Y |
| ProductLine | SIAL |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 10.000000 |
| Lead Time | 9 |
| CAS Number | 68189-42-4 |
| Energy Star | No |
| Green | No |
| Controlled | N |

