Sigma-Aldrich Protein Standard Mix 15 - 600 kDa. for size exclusion chromatography. 30 mg

Name: Sigma-Aldrich Protein Standard Mix 15 - 600 kDa. for size exclusion chromatography. 30 mg
SKU: SIAL-69385-30MG
Price: 244.95 USD

List Price $244.95 Your Price $244.95

Sigma-Aldrich Protein Standard Mix 15 - 600 kDa. for size exclusion chromatography. 30 mg - SIAL (Additional S&H Or Hazmat Fees May Apply)

NETA PART: SIAL-69385-30MG

MFG.PART: 69385-30MG

UNSPSC: 41116107

Manufacturer: Sigma-Aldrich

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Description

Quality Level

100

form

solid

mol wt

15-600 kDa

analyte chemical class(es)

amino acids, peptides, proteins

technique(s)

gel permeation chromatography (GPC): suitable

application(s)

clinical
food and beverages
pharmaceutical

format

neat

storage temp.

−20°C

General description

The protein standard mix is a calibration standard to test and monitor performance of size exclusion chromatography (SEC) columns. It is a lyophilized mixture of molecular weight markers ranging from 15 kDa to 600 kDa.

Components

Thyroglobulin bovine MW ~ 670 000 Da

γ-globulins from bovine blood MW ~ 150 000 Da

Ovalbumin MW~ 44 300 Da

Ribonuclease A type I-A MW ~ 13 700 Da

p-aminobenzoic acid (pABA) MW ~ 137 Da

Application

This analytical standard is used for the following:

Evaluation of selectivity and separation efficiency of size exclusion chromatography (SEC) to separate intact proteins by varying flow rate, size of silica particles and pore sizes in the column
Simultaneous determination of oligomerized and nitrated proteins by size exclusion chromatography-high performance liquid chromatography-diode array detection (SEC-HPLC-DAD)
Molecular weight separation of proteins by size-exclusion chromatography, formed upon O₃ and NO₂ induced oxidation, nitration, and oligomerization of bovine serum albumin (BSA) as a model protein
Estimation of molecular masses of two recombinant proteins— TNF fluorescent sensor (BTN-Kat) and fluorescent sensor-inhibitor (ITN-Kat), by size exclusion chromatography (SEC) to evaluate their ability of binding and neutralizing tumor necrosis factor (TNF) in vitro and further serving as imaging labels for non-invasive analysis

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H302 + H312 + H332,H334,H412

pcodes

P261 - P264 - P273 - P280 - P301 + P312 - P302 + P352 + P312

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c