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Sigma-Aldrich 7-Amino-4-methylcoumarin. ≥98% (HPLC), solid. 10 mg
List Price $115.30 Your Price $115.30
Sigma-Aldrich 7-Amino-4-methylcoumarin. ≥98% (HPLC), solid. 10 mg - SIAL (Additional S&H Or Hazmat Fees May Apply)
NETA PART: SIAL-164545-10MG
MFG.PART: 164545-10MG
UNSPSC: 41116107
Manufacturer: Sigma-Aldrich
Product Name
7-Amino-4-methylcoumarin, Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity.
Quality Level
100
assay
≥98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
yellow
solubility
DMSO: 10 mg/mL
acetone: 10 mg/mL
DMF: soluble
fluorescence
λex 365-380 nm
λem 430-460 nm
shipped in
ambient
General description
Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity. Useful as a reference standard in enzyme assays.
Reagent used to prepare fluorogenic 7-amino-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzymes (excitation maximum: ~365-380 nm; emission maximum: ~430-460 nm). Also useful as a reference standard in enzyme assays.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Detection of proteolytic enzyme activity
Detection of proteolytic enzyme activity
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). DMF and DMSO stock solutions are stable for up to 6 months at -20°C. Acetone stock solutions are stable for up to 1 week at 4°C or -20°C.
Other Notes
Kanaoka, Y., et Al. 1982. Chem. Pharm. Bull. 30, 1485.
Zimmerman, M., et Al. 1977. Anal. Biochem. 78, 47.
Zimmerman, M., et Al. 1977. Anal. Biochem. 78, 47.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
| SKU | SIAL-164545-10MG |
|---|---|
| Supplier Part Number | 164545-10MG |
| UM | EA |
| UNSPSC | 41116107 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIAL |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 10.000000 |
| Lead Time | 9 |
| Energy Star | No |
| Green | No |
| Controlled | N |