Sigma-Aldrich IgG from mouse serum. reagent grade, ≥95% (SDS-PAGE), lyophilized powder. 1 mg

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections[1]
Mouse IgG is purified from normal mouse serum by fractionation and ion-exchange chromatography.

Purified mouse IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, DOT immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Mouse IgG was used as standard in ELISA[2] and chromatin immunoprecipitation assay.[3] It was used to coat nano particles in the development of biosensors.[4][5]

Lyophilized from 0.01 M phosphate buffer, pH 7.2, with 0.015 M NaCl

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