Sigma-Aldrich Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat. affinity isolated antibody, buffered aqueous solution. 1 mL

Immunoglobulin g (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for mouse IgGs.

Purified mouse IgG

Anti-Mouse IgG (whole molecule)-Peroxidase antibody is suitable for use in immunoblot (1:2000). The product can also be used for direct ELISA: 1:10,000.

Western blot analysis of transfected Hela cell lysates was performed using HRP conjugated goat anti-mouse IgG as the secondary at a dilution of 1:4000.[1]

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

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