Revvity HTRF Human Total Cyclin B1 Detection Kit, 50,000 Assay Points

The Total Cyclin B1 cell-based assay provides a convenient and accurate way of detecting total Cyclin B1 levels within cells. This kit is compatible with buffers from the phospho-Cyclin B1 (Ser126) kit, SO the same lysate can be used for analyses of both phosphorylated and total protein populations.

Cyclin B family members (including Cyclin B1 and Cyclin B2) are important regulators of cell cycle progression. They are integral mediators of the growth factor-dependent transition from G2 to Mitosis phases, playing the role of the regulatory subunit of CDK1.

Cyclin B1 is upregulated in the presence of extracellular mitogenic stimuli and is subsequently rapidly downregulated by proteasomal degradation following its phosphorylation on Ser126.

Cyclin B1 is often overexpressed in many cancers. Such abnormal overexpression results in dysregulated CDK1 activity and can lead to tumorigenesis.