Revvity HTRF Human Total DAP12 Detection Kit, 50,000 assay points

The Total DAP12 cellular assay monitors total DAP12 protein levels and can be used as a normalization assay in conjunction with our Phospho-DAP12 (Tyr91) kit. This kit is designed to be compatible with the buffers from the Phospho-DAP12 kit, allowing the same lysate to be used for analyses of both the phosphorylated and the total protein populations. DAP12 (DNAX-Activation Protein 12), also known as TYROBP (TYRO protein tyrosine kinase-binding protein), is a transmembrane signaling adaptor protein that plays a crucial role in innate immune responses and neuroinflammation. DAP12 is widely expressed in various immune cells, including natural killer cells, monocytes, macrophages, and microglia, the resident immune cells of the central nervous system. The DAP12-TREM2 signaling axis is particularly important in the context of neuroinflammation and neurodegenerative diseases. It regulates microglial activation and function, influencing the brains immune response and homeostasis. Genetic variants of TREM2 have been identified as risk factors for Alzheimers Disease, highlighting the significance of this pathway in maintaining brain health. Monitoring total DAP12 levels alongside its phosphorylated form provides valuable insights into the overall expression and activation state of this crucial signaling protein.