Revvity pHSense Eu SNAP Labeling Reagent, 10 x 96 wells

The pHSense™ Eu SNAP Labeling Reagent is non cell permeant, and is labeled with a pH sensitive europium complex that specifically and covalently interacts with SNAP-tag® receptors and membrane proteins to monitor their internalization. Cells expressing receptors and membrane proteins tagged with a SNAP-tag on the extracellular part can be labeled with pHSense™ Eu SNAP Labeling Reagent and remain minimally fluorescent at neutral extracellular pH (≥7). Once internalized, the pHSense™ Eu SNAP Labeling Reagent encounters increasingly acidic compartments such as early and late endosomes and lysosomes, where the europium signal becomes progressively stronger. This new pH sensitive europium SNAP-labeling reagent is compatible with a time-resolved fluorescence (TRF) detection, effectively eliminating most fluorescence background and significantly enhancing the signal-to-background ratio. Its unique photophysical properties enable simple and robust no-wash detection of receptor-mediated endocytosis in plate-based assays with live-cells.