Revvity AlphaLISA <em>SureFire Ultra</em> Human Phospho-Ret (Tyr905) Detection Kit, 50,000 assay points

Rearranged during transfection (Ret) is a receptor tyrosine kinase that governs cell growth, survival, and differentiation. Upon activation by glial cell line-derived neurotrophic factor (GDNF) family ligands, Ret triggers downstream signaling cascades, including MAPK, PI3K/AKT, and JAK/STAT. Ret fusions and activating mutations drive oncogenesis in thyroid cancer, lung adenocarcinoma, and multiple endocrine neoplasia (MEN) syndromes, making it a key target for precision therapies.

The AlphaLISA SureFire Ultra Human Phospho-Ret (Tyr905) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Ret (Tyr905) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies