Revvity AlphaLISA SureFire Biotin-Free Human and Mouse Phospho-STAT5 (Tyr694/699) Detection Kit, 50,000 Assay Points

STAT5, comprising STAT5A and STAT5B, is activated by JAK kinases in response to cytokine signaling. Upon phosphorylation, STAT5 forms dimers and translocates to the nucleus, inducing transcription of genes involved in proliferation, differentiation, and survival. Overactive STAT5 drives cancers like leukemia and breast cancer, while its dysregulation contributes to autoimmune diseases by promoting excessive immune responses.

The AlphaLISA SureFire Biotin-Free Human and Mouse Phospho-STAT5 (Tyr694/699) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-STAT5 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Biotin Free kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies
• Biotin rich samples

AlphaLISA SureFire Biotin Free kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies