Revvity AlphaLISA SureFire Biotin-Free Human and Mouse Phospho-Rb (Thr826) Detection Kit, 50,000 Assay Points

The retinoblastoma protein (Rb), encoded by RB1, is a tumor suppressor that regulates the cell cycle by inhibiting E2F transcription factors, thereby blocking S phase entry. Rb is inactivated via phosphorylation by cyclin-dependent kinases, enabling cell cycle progression. Mutations in RB1 cause retinoblastoma and are implicated in various cancers, including osteosarcoma and lung cancer, due to the loss of cell cycle control.

The AlphaLISA SureFire Biotin-Free Human and Mouse Phospho-Rb (Thr826) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Rb in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Biotin Free kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies
• Biotin rich samples

AlphaLISA SureFire Biotin Free kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies