Revvity AlphaLISA SureFire Biotin-Free Human and Mouse Total STAT3 Detection Kit, 50,000 Assay Points

STAT3 is a transcription factor activated by cytokine and growth factor signaling, particularly through JAK2 or receptor tyrosine kinases. Once phosphorylated, STAT3 dimerizes and regulates genes governing cell proliferation, survival, and immune responses. Constitutive STAT3 activation is implicated in cancers, such as breast and lung cancers, promoting tumor growth and metastasis, as well as autoimmune and neurodegenerative diseases.

The AlphaLISA SureFire Biotin-Free Human and Mouse Total STAT3 Detection Kit is a sandwich immunoassay for the quantitative detection of total STAT3 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Biotin Free kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies
• Biotin rich samples

AlphaLISA SureFire Biotin Free kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies