Revvity AlphaLISA <em>SureFire</em> Biotin-Free Human Total SYK Detection Kit, 50,000 Assay Points

Spleen tyrosine kinase (SYK) is a non-receptor kinase crucial for immune cell signaling, particularly in B cells and microglia. Activated through interaction with phosphorylated ITAM motifs, SYK triggers downstream pathways governing cell activation, proliferation, and survival. SYK acts as both a tumor suppressor and promoter, depending on the cancer type, and is a key driver of autoimmune diseases like rheumatoid arthritis and lupus.

The AlphaLISA SureFire Biotin-Free Human Total SYK Detection Kit is a sandwich immunoassay for the quantitative detection of total SYK in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Biotin Free kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies
• Biotin rich samples

AlphaLISA SureFire Biotin Free kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies