Revvity AlphaLISA <em>SureFire</em> Biotin-Free Human Phospho-CDK1 (Thr14) Detection Kit, 50,000 Assay Points

Cyclin-dependent kinase 1 (CDK1) is a serine/threonine kinase critical for cell cycle regulation. It partners with cyclin B to control the G2/M transition, ensuring proper mitotic progression through phosphorylation of key substrates. CDK1 overexpression is common in cancers, such as breast and lung cancers, promoting uncontrolled proliferation. Dysregulated CDK1 activity is also linked to neurodegenerative diseases like Alzheimers, where it contributes to neuronal cell death.

The AlphaLISA SureFire Biotin-Free Human Phospho-CDK1 (Thr14) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-CDK1 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Biotin Free kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies
• Biotin rich samples

AlphaLISA SureFire Biotin Free kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies