Revvity AlphaLISA <em>SureFire</em> Biotin-Free Human Phospho-STAT6 (Tyr641) Detection Kit, 50,000 Assay Points

STAT6 is a transcription factor activated primarily by interleukin-4 (IL-4) signaling, leading to phosphorylation at tyrosine 641. STAT6 dimers regulate genes involved in Th2 immune responses, promoting anti-apoptotic activity and IgE production. Dysregulation of STAT6 is linked to allergic diseases, including asthma and atopic dermatitis, as well as certain cancers, where it supports tumor survival and proliferation.

The AlphaLISA SureFire Biotin-Free Human Phospho-STAT6 (Tyr641) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-STAT6 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Biotin Free kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies
• Biotin rich samples

AlphaLISA SureFire Biotin Free kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies