Revvity AlphaLISA <em>SureFire Ultra</em> Human Total c-Kit Detection Kit, 50,000 Assay Points

c-KIT, also known as CD117, is a receptor tyrosine kinase activated by stem cell factor (SCF). Ligand binding triggers receptor dimerization, autophosphorylation, and activation of downstream pathways, including MAPK, PI3K/AKT, and JAK/STAT. Dysregulated c-KIT signaling drives tumor proliferation and survival in gastrointestinal stromal tumors (GIST), leukemia, and melanoma.

The AlphaLISA SureFire Ultra Human Total c-Kit Detection Kit is a sandwich immunoassay for the quantitative detection of total c-Kit in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies