Revvity AlphaLISA <em>SureFire Ultra</em> Human Total AXIN-2 Detection Kit, 50,000 Assay Points

AXIN-2 is a scaffold protein crucial for the WNT/β-catenin signaling pathway, promoting β-catenin degradation by assembling the destruction complex with APC, GSK3β, and CK1α. Dysregulation of AXIN-2 disrupts β-catenin levels, driving cancers such as colorectal cancer. Loss of AXIN-2 function is also observed in breast cancer and neuroblastoma.

The AlphaLISA SureFire Ultra Human Total AXIN-2 Detection Kit is a sandwich immunoassay for the quantitative detection of total AXIN-2 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies