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Cell Signaling PathScan® RP Cleaved Gasdermin D (Gly277) Sandwich ELISA Kit; 1 Kit
List Price
$660.54
Your Price
$660.54
Cell Signaling PathScan® RP Cleaved Gasdermin D (Gly277) Sandwich ELISA Kit; 1 Kit - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-60707C
MFG.PART:
60707C
UNSPSC:
41116158
Manufacturer:
Cell Signaling
Product Description
The rapid protocol (RP) PathScan ® RP Cleaved Gasdermin D (Gly277) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of the carboxy-terminal fragment of Gasdermin D protein when cleaved at Gly277 in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with cleaved Gasdermin D (Gly277) in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of cleaved Gasdermin D (Gly277). Learn more about your ELISA kit options here .
*Antibodies in this kit are custom formulations specific to kit.
Specificity / Sensitivity
The PathScan ® RP Cleaved Gasdermin D (Gly277) Sandwich ELISA Kit detects endogenous levels of the carboxy-terminal fragment of mouse Gasdermin D protein when cleaved at Gly277. This kit sensitivity is shown in Figures 1 and 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Mouse
Product Specifications
| REACTIVITY | M |
Application Key:
- ELISA -ELISA
Species Cross-Reactivity Key:
- M -Mouse
| Product Includes | Volume | Solution Color |
|---|---|---|
| Gasdermin D Rabbit mAb Coated Microwells #93727 | 96 tests | |
| Cleaved Gasdermin D (Gly277) Rabbit Detection mAb #85488 | 1 ea | Red (Lyophilized) |
| HRP Diluent #13515 | 5.5 mL | Red |
| TMB Substrate #7004 | 11 mL | |
| STOP Solution #7002 | 11 mL | |
| Sealing Tape #54503 | 2 ea | |
| ELISA Wash Buffer (20X) #9801 | 25 mL | |
| Cell Lysis Buffer (10X) #9803 | 15 mL |
Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.
For the “C” and “V” kits, the supplied 96-well strip plate consists of twelve 8-well strips in a support frame. This enables custom plate configurations.
| SKU | CSIG-60707C |
|---|---|
| Supplier Part Number | 60707C |
| UM | EA |
| UNSPSC | 41116158 |
| Manufacturer | Cell Signaling |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |