RevvitypHSense Eu Anti-FLAG, 2 x 96 wells

pHSense™ Eu Anti-FLAG is non cell permeant, and is labeled with a pH sensitive europium complex. This Monoclonal Anti-FLAG antibody is designed to monitor receptor and membrane protein internalization when tagged with the FLAG® sequence (DYKDDDDK) on the extracellular part. The antibody recognizes the FLAG® sequence fused at the N-terminus, intra or C-terminus and remains minimally fluorescent at neutral extracellular pH (≥7). Once internalized, the pHSense™ Eu Anti-FLAG encounters increasingly acidic compartments such as early and late endosomes and lysosomes, where the europium signal becomes progressively stronger. This new pH sensitive europium anti-FLAG is compatible with a time-resolved fluorescence (TRF) detection, effectively eliminating most fluorescence background and significantly enhancing the signal-to-background ratio. Its unique photophysical properties enable simple and robust no-wash detection of receptor-mediated endocytosis in plate-based assays with live-cells.