RevvityAlphaLISA <em>SureFire Ultra</em> Human Phospho-CSF1R (Tyr723) Detection Kit, 50,000 Assay Points

Colony Stimulating Factor 1 Receptor (CSF1R) is a receptor tyrosine kinase primarily expressed on monocytes, macrophages, and microglia. It binds two primary ligands CSF1 (M-CSF) and IL-34, promoting survival, proliferation, and differentiation of these immune cells. CSF1R signaling is essential for tissue homeostasis, inflammation, and innate immune responses. Aberrant CSF1R activity is associated with cancer progression, immune evasion, and neurodegenerative disorders. In oncology, CSF1R inhibitors aim to reprogram tumor-associated macrophages (TAMs) and enhance anti-tumor immunity. In the CNS, CSF1R is a therapeutic target in diseases like Alzheimers and ALS due to its role in microglial activation and neuroinflammation.

The AlphaLISA SureFire Ultra Human Phospho-CSF1R (Tyr723) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-CSF1R (Tyr723) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies