RevvityAlphaLISA <em>SureFire Ultra</em> Human and Mouse Total CHOP Detection Kit, 50,000 Assay Points

C/EBP Homologous Protein (CHOP), also known as DDIT3, is a stress-inducible transcription factor primarily activated during endoplasmic reticulum (ER) stress. It is primarily activated through the unfolded protein response (UPR), particularly downstream of the PERK–eIF2α–ATF4 signaling axis. CHOP promotes apoptosis when ER stress is severe or prolonged, by upregulating pro-apoptotic genes, repressing anti-apoptotic factors such as BCL2, and modulating oxidative stress pathways. Elevated CHOP expression is implicated in various diseases, including neurodegeneration, metabolic disorders, and cancer. In tumors, CHOP can either suppress or promote tumorigenesis depending on the context, making it a nuanced target for therapeutic intervention.

The AlphaLISA SureFire Ultra Human and Mouse Total CHOP Detection Kit is a sandwich immunoassay for the quantitative detection of total CHOP in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies