RevvityPhenoVue Astrocyte Differentiation Staining Kit (1 x 384-wells)

The PhenoVue astrocyte differentiation staining kit offers a reliable solution for confirming astrocyte phenotype through a multiplexed staining approach.

This ready-to-use kit enables the visualization and qualification of iPSC-derived astrocytes by combining a well-characterized antibody against GFAP (glial fibrillary acidic protein), the gold-standard astrocyte marker, with actin cytoskeleton and nuclear stains to assess cell morphology and organization.

The PhenoVue Astrocyte differentiation staining kit includes three fluorescent markers:

• GFAP: Glial fibrillary acidic protein is an intermediate filament protein highly expressed in mature astrocytes and widely used to distinguish astrocytes from other CNS cell types.
• Phalloidin (conjugated to PhenoVue Fluor 555): provides detailed cytoskeletal visualization for morphology assessment.
• PhenoVue Hoechst 33342: nuclei staining for cell identification and quantification.

Each component of the kit has been carefully tested and optimized, and designed to allow for spectral separation with minimal overlap, even when adding additional fluorescent stains, such as those compatible with the PhenoVue Fluor 647 channel, to measure other proteins or cellular events in parallel. Furthermore, the specificity of each reagent has been confirmed, making this kit a reliable and easy-to-use solution for qualifying astrocyte differentiation and assessing activation states based on cellular morphology.

Kit components/quantity

• PhenoVue Hoechst 33342 nuclear stain / 1 vial of 70 μL (500x)
• PhenoVue Fluor 555 - Phalloidin / 1 vial of 60 μL (600x)
• PhenoVue anti-GFAP mouse IgG1 antibody / 1 vial of 100 μL (100x)
• PhenoVue Fluor 488 - Goat anti-mouse antibody, highly cross-adsorbed / 1 vial of 100 μL (100x)
• PhenoVue Paraformaldehyde, 4% solution / 1 vial of 25 mL (1x)
• PhenoVue Permeabilization, 0.5% Triton X-100 solution / 1 vial of 25 mL (5x)
• PhenoVue Dye diluent A / 1 vial of 8 mL (5x)