RevvityPhenoVue anti-53BP1 - Rat IgG2a Antibody, 100x

DNA damage ranges from single-strand breaks (SSBs) to double-strand breaks (DSBs) and complex base modifications. DSBs are the most harmful, leading to genomic instability or cell death if unrepaired. Cells activate the DNA damage response (DDR) to detect and repair damage which is crucial for studying genome stability, assessing cytotoxic agents, and developing anticancer drugs. DSBs trigger the relocalization of key DDR proteins (MRE11/NBS1/RAD50, MDC1, 53BP1, BRCA1) to nuclear foci, where they interact with γ-H2AX. 53BP1 promotes canonical non-homologous end joining (C-NHEJ) over homologous recombination and alternative NHEJ.

The PhenoVue anti-53BP1 – rat IgG2a antibody, part of the PhenoVue DNA Damage Response Staining Kit (PDDR11), supports imaging and high-content analysis applications.