Revvity pHSense Eu SNAP Labeling Reagent, 96 wells

The pHSense Eu SNAP Labeling Reagent is non cell permeant, and is labeled with a pH sensitive europium complex that specifically and covalently interacts with SNAP-tag receptors and membrane proteins to monitor their internalization. Cells expressing receptors and membrane proteins tagged with a SNAP-tag on the extracellular part can be labeled with pHSense Eu SNAP Labeling Reagent and remain minimally fluorescent at neutral extracellular pH (≥7). Once internalized, the pHSense Eu SNAP Labeling Reagent encounters increasingly acidic compartments such as early and late endosomes and lysosomes, where the europium signal becomes progressively stronger. This new pH sensitive europium SNAP-labeling reagent is compatible with a time-resolved fluorescence (TRF) detection, effectively eliminating most fluorescence background and significantly enhancing the signal-to-background ratio. Its unique photophysical properties enable simple and robust no-wash detection of receptor-mediated endocytosis in plate-based assays with live-cells.