Revvity pHSense Eu Anti-FLAG, 2 x 96 wells

pHSense Eu Anti-FLAG is non cell permeant, and is labeled with a pH sensitive europium complex. This Monoclonal Anti-FLAG antibody is designed to monitor receptor and membrane protein internalization when tagged with the FLAG sequence (DYKDDDDK) on the extracellular part. The antibody recognizes the FLAG sequence fused at the N-terminus, intra or C-terminus and remains minimally fluorescent at neutral extracellular pH (≥7). Once internalized, the pHSense Eu Anti-FLAG encounters increasingly acidic compartments such as early and late endosomes and lysosomes, where the europium signal becomes progressively stronger. This new pH sensitive europium anti-FLAG is compatible with a time-resolved fluorescence (TRF) detection, effectively eliminating most fluorescence background and significantly enhancing the signal-to-background ratio. Its unique photophysical properties enable simple and robust no-wash detection of receptor-mediated endocytosis in plate-based assays with live-cells.