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Sigma-Aldrich Wst-1
List Price $176.32 Your Price $176.32
Sigma-Aldrich Wst-1 - SIAL (Additional S&H or Hazmat Fees May Apply)
NETA PART: SIAL-SBR00125-100MG
MFG.PART: SBR00125-100MG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Quality Level
300
assay
≥90% (HPLC)
form
solid
application(s)
cell analysis
detection
storage temp.
−20°C (−15°C to −25°C)
General description
WST-1(water-soluble tetrazolium salt 1) is a potent reagent for evaluating cellproliferation, growth, and chemosensitivity in various cell populations. Thiscompound converts a stable tetrazolium salt into a soluble formazan dye at thecell surface, with dye production directly correlating to the number of viable,metabolically active cells. To enable electron transfer from intracellularreducing agents to WST-1, an electron coupling reagent like 1-methoxy phenazinemethosulfate (PMS) is often required, facilitating a colorimetric assay forcell viability.
Application
- WST-1 is optimized for 96-well plate formats, allowing simultaneous processing of multiple samples.
- Highly sensitive making it suitable for measuring cell proliferation in response to growth factors and nutrients, as well as assessing the impact of growth-inhibitory antibodies and cytotoxic compounds, including anti-cancer drugs.
- WST-1 can monitor enzymatic reactions by utilizing NADH/NADPH electron transfer to PMS, applicable for measuring dehydrogenase activity or other electron transfer reactions.
| SKU | SIAL-SBR00125-100MG |
|---|---|
| Supplier Part Number | SBR00125-100MG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIAL |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| CAS Number | 150849-52-8 |
| Energy Star | No |
| Green | No |
| Controlled | N |