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Sigma-Aldrich Dna2 Inhibitor C5
List Price $103.24 Your Price $103.24
Sigma-Aldrich Dna2 Inhibitor C5 - SIAL (Additional S&H or Hazmat Fees May Apply)
NETA PART: SIAL-SML4192-25MG
MFG.PART: SML4192-25MG
UNSPSC: 12352200
Manufacturer: Sigma-Aldrich
Quality Level
100
assay
≥95% (HPLC)
form
powder
color
white to beige
solubility
DMSO: 2 mg/mL, clear (warmed)
storage temp.
2-8°C
SMILES string
O=C(C1=CN=C2C(C=CC=C2[N+]([O-])=O)=C1O)O
Application
DNA2 Inhibitor C5 may be used to evaluate the on-target effects on DNA2 in cancer cells.
Biochem/physiol Actions
DNA2 Inhibitor C5 suppresses nuclease/helicase activities, impairs DNA repair/replication, causes cell cycle arrest, and shows synergistic cancer cell killing with DNA-damaging agents and PARP/ATR inhibitors.
The DNA2 inhibitor C5 (DNA2i-C5) binds to a pocket near the DNA binding site in the helicase domain of DNA2 with an IC50 of 20-30 μM for inhibiting nuclease activity and DNA binding. C5 effectively suppresses both the nuclease and helicase activities of DNA2, impairing DNA end resection, homologous recombination, single-strand annealing repair, and replication fork restart after stalling. In cells, C5 mimics genetic knockout of DNA2, causing cell cycle arrest in late S/G2 phase, reducing mitotic entry, and leading to chromosomal segregation defects, particularly affecting centromeric DNA replication and integrity. Importantly, C5 shows synergistic killing of various cancer cell types when combined with DNA damaging agents like camptothecin or inhibitors of PARP and ATR, highlighting its potential as a cancer therapeutic strategy.
The DNA2 inhibitor C5 (DNA2i-C5) binds to a pocket near the DNA binding site in the helicase domain of DNA2 with an IC50 of 20-30 μM for inhibiting nuclease activity and DNA binding. C5 effectively suppresses both the nuclease and helicase activities of DNA2, impairing DNA end resection, homologous recombination, single-strand annealing repair, and replication fork restart after stalling. In cells, C5 mimics genetic knockout of DNA2, causing cell cycle arrest in late S/G2 phase, reducing mitotic entry, and leading to chromosomal segregation defects, particularly affecting centromeric DNA replication and integrity. Importantly, C5 shows synergistic killing of various cancer cell types when combined with DNA damaging agents like camptothecin or inhibitors of PARP and ATR, highlighting its potential as a cancer therapeutic strategy.
| SKU | SIAL-SML4192-25MG |
|---|---|
| Supplier Part Number | SML4192-25MG |
| UM | EA |
| UNSPSC | 12352200 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIAL |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| CAS Number | 35973-25-2 |
| Energy Star | No |
| Green | No |
| Controlled | N |