Sigma-Aldrich Anti-Trpv2 Clone 1G5 Zoomab Rabbit

Transient receptor potential cation channel subfamily V member 2 (UniProt: Q9Y5S1; also known as Osm-9-like TRP channel 2 (OTRPC2), Vanilloid receptor-like protein 1 (VRL-1)) is encoded by the TRPV2 (also known as VRL) gene (Gene ID: 51393) in human. TrpV2 is an ubiquitously expressed, calcium-permeable, non-selective cation channel with an outward rectification. It has been implicated in various physiological and pathological processes, including growth factor signaling, macrophage migration, cancer cell metastasis, heart disease, and neuronal cell development. It appears to be regulated by growth factors, like IGF1, PDGF, and morphogenetic neuropeptide/head activator. It is also activated by temperatures higher than 52ºC, but is not activated by vanilloids and acidic pH. TrpV2 channel is upregulated by the nerve growth factor (NGF) via the mitogen-activated protein kinase (MAPK) signaling pathway to augment neurite outgrowth. Although NGF increases TrpV2 content, it does not induce the translocation of TrpV2 from cytoplasm to cell surface in PC12 cells. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Cohen MR et Al. (2015). Mol. Cell Biol. 35(24): 4238-4252).

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal cytoplasmic domain of human TRPV2.

Quality Control Testing

Evaluated by Western Blotting in human MDA-MB-435 cells lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TRPV2 in human MDA-MB-435 cells lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TRPV2 in lysate from human dorsal root ganglion (DRG) and 1:10,000 dilution was used on human tonsil tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TRPV2 in human dorsal root ganglion tissue sections.

Immunocytochemistry Analysis: A 1:1,000 Dilution from a representative lot detected TRPV2 in HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1G5 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects TRPV2. It targets an epitope within 25 amino acids from the C-terminal cytoplasmic domain.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.