Sigma-Aldrich Anti-BSA Clone 3H2 Zoomab Rb Mono

Albumin (UniProt: P02769; also known as BSA, Bos d 6) is encoded by the ALB gene (Gene ID: 280717) in bovine. Albumin is the most abundant circulating proteins present in the serum that serves as a carrier for various endogenous and exogenous substances, including fatty acids, hormones, bilirubin, and drugs. Albumin is a globular protein arranged in three homologous domains. Each domain contains multiple alpha-helices, contributing to the protein′s stability and solubility. It contains multiple binding sites, which allow it to interact with a wide range of molecules. It plays a crucial role in maintaining colloidal osmotic pressure and transporting nutrients and metabolites throughout the body. Its ability to bind and transport zinc makes it a major zinc transporter in plasma, typically binding about 80% of all plasma zinc. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of Bovine Serum Albumin (BSA).

Quality Control Testing

Evaluated by Western Blotting with purified bovine serum albumin (BSA).

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected purified bovine serum albumin (BSA).

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected BSA in purified phosphorylcholine derivatives of bovine serum albumin (PC-BSA).

Affinity Binding Assay: A representative lot of this antibody bound with BSA peptide with at least ten thousand-fold (10,000X) higher affinity than with non-specific control peptide.

Enzyme Immunoassay: A representative lot of this antibody detected BSA in ELISA application.

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary with the end user.

Clone 3H2 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Bovine Serum Albumin (BSA). It targets an epitope within 19 amino acids from the N-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.